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L.M. KOVAL, O.N. YAVORSKA, E.A. LUKYANETZ (2020) 'LIPID DROPLETS IN CALCIUM-INDUCED STRUCTURE INTERACTIONS IN ADRENOCORTICOCYTES' in O.A. Krishtal, E.A. Lukyanetz (Eds.), ESSAYS ON NEUROPHYSIOLOGY BY PLATON KOSTYUK AND HIS STUDENTS, AKADEMPERIODYKA, pp. 210-216

LIPID DROPLETS IN CALCIUM-INDUCED STRUCTURE INTERACTIONS IN ADRENOCORTICOCYTES

L.M. KOVAL, O.N. YAVORSKA, E.A. LUKYANETZ

    Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/biph.books.EssNeur.210


Abstract

Intracellular calcium signaling plays a key role in steroidogenesis in adrenocortical cells. While zona glomerulosa (ZG) cells rely on calcium influx for hormone synthesis, the role of calcium in zona fasciculata (ZF) and zona reticularis (ZR) cells remains less clear. Using ultrastructural analysis and calcium imaging, we investigated calcium-induced interactions between lipid droplets and mitochondria in ZF/ZR cells. Membrane depolarization via potassium chloride (KCl) significantly increased intracellular calcium concentration [Ca2+]i and led to the formation of specialized lipid droplet structures (SLB) at organelle contact sites, a phenomenon not observed with ionophore-induced calcium influx. These findings suggest that spatially confined calcium entry plays a crucial role in steroidogenesis by regulating intracellular lipid droplet-mitochondria interactions, potentially influencing steroid hormone synthesis.

Keywords: calcium signaling, steroidogenesis, zona fasciculata, zona reticularis, lipid droplets, mitochondria, intracellular interactions, membrane depolarization, adrenocortical cells, hormone synthesis

Full text: (PDF, in English)

References

  1. Aptel HB, Johnson EI, Vallotton MB, Rossier MF, Capponi AM. 1996. Demonstration of an angiotensin II-induced negative feedback effect on aldosterone synthesis in isolated rat adrenal zona glomerulosa cells. Mol Cell Endocrinol 119: 105-111. CrossRef PubMed
  2. Barbara JG, Takeda K. 1995. Voltage-dependent currents and modulation of calcium channel expression in zona fasciculata cells from rat adrenal gland. J Physiol 488(Pt 3): 609-622. CrossRef PubMed PubMedCentral
  3. Belloni AS, Mazzocchi G, Mantero F, Nussdorfer GG. 1987. The human adrenal cortex: ultrastructure and base-line morphometric data. J Submicrosc Cytol 19: 657-668.
  4. Bornstein SR, Ehrhart-Bornstein M, Guse-Behling H, Scherbaum WA. 1992. Structure and dynamics of adrenal mitochondria following stimulation with corticotropin releasing hormone. Anat Rec 234: 255-262. CrossRef PubMed
  5. Brandenburger Y, Kennedy ED, Python CP, Rossier MF, Vallotton MB, Wollheim CB, Capponi AM. 1996. Possible role for mitochondrial calcium in angiotensin II- and potassium-stimulated steroidogenesis in bovine adrenal glomerulosa cells. Endocrinology 137: 5544-5551. CrossRef PubMed
  6. Brasaemle DL, Levin DM, Adler-Wailes DC, Londos C. 2000. The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surfaces of lipid storage droplets. Biochim Biophys Acta 1483: 251-262. CrossRef
  7. Capponi AM, Rossier MF, Davies E, Vallotton MB. 1988. Calcium stimulates steroidogenesis in permeabilized bovine adrenal cortical cells. J Biol Chem 263: 16113-16117. CrossRef PubMed
  8. Cherradi N, Brandenburger Y, Rossier MF, Capponi AM. 1998. Regulation of mineralocorticoid biosynthesis by calcium and the StAR protein. Endocr Res 24: 355-362. CrossRef PubMed
  9. Cote M, Guillon G, Payet MD, Gallo-Payet N. 2001. Expression and regulation of adenylyl cyclase isoforms in the human adrenal gland. J Clin Endocrinol Metab 86: 4495-4503. CrossRef PubMed
  10. Coyne MD, Wang G, Lemos JR. 1996. Calcium channels do not play a role in the steroid response to ACTH in Y1 adrenocortical cells. Endocr Res 22: 551-556. CrossRef PubMed
  11. Ishimura K, Fujita H. 1997. Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes. Microsc Res Tech 36: 445-453. CrossRef
  12. Kim YC, Ariyoshi N, Artemenko I, Elliott ME, Bhattacharyya KK, Jefcoate CR. 1997. Control of cholesterol access to cytochrome P450scc in rat adrenal cells mediated by regulation of the steroidogenic acute regulatory protein. Steroids 62: 10-20. CrossRef PubMed
  13. Koval LM, Yavorskaya EN, Lukyanetz EA. 2001. Electron microscopic evidence for multiple types of secretory vesicles in bovine chromaffin cells. Gen Comp Endocrinol 121: 261-277. CrossRef PubMed
  14. Koval LM, Yavorskaya EN, Tokar SL, Lukyanetz EA. 2000. Ultrastructural properties of steroid vesicles and mitochondria in bovine adrenocortical cells. Neurophysiology (Kyiv) 32: 272-274. CrossRef
  15. Lukyanetz EA. 2001. Intracellular events during depolarization-induced exocytosis in chromaffin cells. In: Morad M, Kostyuk P (eds.) Calcium Signalling, vol. 331. IOS Press, Amsterdam, pp. 174-182.
  16. Lukyanetz EA, Neher E. 1999. Different types of calcium channels and secretion from bovine chromaffin cells. Eur J Neurosci 11: 2865-2873. CrossRef PubMed
  17. McGookey DJ, Anderson RG. 1983. Morphological characterization of the cholesteryl ester cycle in cultured mouse macrophage foam cells. J Cell Biol 97: 1156-1168. CrossRef PubMed PubMedCentral
  18. Morimoto C, Kiyama A, Kameda K, Ninomiya H, Tsujita T, Okuda H. 2000. Mechanism of the stimulatory action of okadaic acid on lipolysis in rat fat cells. J Lipid Res 41: 199-204. CrossRef PubMed
  19. Rossier MF. 1997. Confinement of intracellular calcium signaling in secretory and steroidogenic cells. Eur J Endocrinol 137: 317-325. CrossRef PubMed
  20. Rossier MF, Burnay MM, Brandenburger Y, Cherradi N, Vallotton MB, Capponi AM. 1996a. Sources and sites of action of calcium in the regulation of aldosterone biosynthesis. Endocr Res 22: 579-588. CrossRef PubMed
  21. Rossier MF, Burnay MM, Vallotton MB, Capponi AM. 1996b. Distinct functions of T- and L-type calcium channels during activation of bovine adrenal glomerulosa cells. Endocrinology 137: 4817-4826. CrossRef PubMed
  22. Schrier AD, Wang H, Talley EM, Perez-Reyes E, Barrett PQ. 2001. Alpha1H T-type Ca²⁺ channel is the predominant subtype expressed in bovine and rat zona glomerulosa. Am J Physiol Cell Physiol 280: C265-C272. CrossRef PubMed
  23. van Meer G. 2001. Caveolin, cholesterol, and lipid droplets? J Cell Biol 152: F29-F34. CrossRef PubMed PubMedCentral
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