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T. ZHELAI, S. ARUNACHALAM, D.R. GIOVANNUCCI (2020) 'VOLTAGE-OPERATED AND NON-VOLTAGE-OPERATED Ca2+ ENTRY PATHWAYS IN GASTROENTEROPANCREATIC NEUROENDOCRINE TUMOR CELL LINES' in O.A. Krishtal, E.A. Lukyanetz (Eds.), ESSAYS ON NEUROPHYSIOLOGY BY PLATON KOSTYUK AND HIS STUDENTS, AKADEMPERIODYKA, pp. 285-292

VOLTAGE-OPERATED AND NON-VOLTAGE-OPERATED Ca2+ ENTRY PATHWAYS IN GASTROENTEROPANCREATIC NEUROENDOCRINE TUMOR CELL LINES

T. ZHELAI1,2, S. ARUNACHALAM2, D.R. GIOVANNUCCI2

  1. Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine;
  2. University of Toledo College of Medicine, Toledo,USA
DOI: https://doi.org/10.15407/biph.books.EssNeur.285


Abstract

The role for Ca2+ in cancer-related cell signaling pathways is well established. Alterations in Ca2+ homeostasis increase proliferation and induce dierentiation and apoptosis. According to a growing number of studies, Ca2+ channels voltage- and nonvoltage-gated family represents key players in calcium homeostasis and cell physiopathology. We proposed that Ca2+ entry through plasma membrane channels could provide an additional or alternative pathway for modulation of cell growth in gut neuroendocrine cells. To investigate this possibility, we characterized Ca2+ entry in a set of human carcinoid cell lines originating in the foregut, midgut and hindgut as a starting point for an inquiry into the role of Ca2+ signaling pathways in carcinoid cancer. To test this hypothesis, we used RT-PCR to profile a variety of voltage-operated and non-voltage-operated Ca2+ permeable channels and then characterized VOCE and SOCE in the carcinoid cell lines.

Keywords: Calcium signaling, voltage-operated calcium channels (VOCC), store-operated calcium entry (SOCE), transientTRP channels, carcinoid tumor, neuroendocrine cells, ion channels, enteroendocrine system, epithelial-to-neuroendocrine transition, tumor proliferation, cell migration, calcium homeostasis, NFAT, CREB, cancer therapeutics.

Full text: (PDF, in English)

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