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S.M. MARCHENKO, O.A. FEDORENKO, V.V. YAROTSKY, D.E. DUZHYI (2020) 'PROPERTIES OF NEURONAL INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS/ Ca2+-RELEASE CHANNELS' in O.A. Krishtal, E.A. Lukyanetz (Eds.), ESSAYS ON NEUROPHYSIOLOGY BY PLATON KOSTYUK AND HIS STUDENTS, AKADEMPERIODYKA, pp. 267-271

PROPERTIES OF NEURONAL INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS/ Ca2+-RELEASE CHANNELS

S.M. MARCHENKO1, O.A. FEDORENKO1,2, V.V. YAROTSKY1,3, D.E. DUZHYI1

  1. Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine;
  2. Universitй de Picardie Jules Verne, Amiens, France
  3. Penn State College of Medicine, Hershey, USA
DOI: https://doi.org/10.15407/biph.books.EssNeur.267


Abstract

Inositol 1,4,5-trisphosphate receptors (InsP3Rs) are a major type of intracellular Ca2+ release channels localized redominantly in the endoplasmic reticulum. Notwith-standing numerous investigations, there is a significant controversy on the basic properties of these receptors. There is also a long-standing controversy on the role of the nuclear envelope in the regulation of nuclear Ca2+. To address these issues, we have studied native InsP3Rs in the nuclear membrane of mammalian cells. The nuclear envelope is the only part of the endoplasmic reticulum readily accessible for patch-clamp recording. Very high levels of expression of InsP3Rs were reported for some types of neurons. Investigation of neuronal nuclei is hampered by the cellular heterogeneity of the brain where neurones comprise no more than 10% of the cells. We have developed methods for isolation of cell nuclei from identified types of central neurons . Here we report some recent data on the properties of neuronal InsP3Rs.

Keywords: Inositol 1,4,5-trisphosphate receptors (InsP3Rs), calcium signaling, nuclear membrane, Purkinje neurons, hippocampal pyramidal neurons, patch-clamp electrophysiology, Ca²⁺-dependent desensitization, intracellular Ca²⁺ release, transient Ca²⁺ signals, endoplasmic reticulum, neuronal excitability.

Full text: (PDF, in English)

References

  1. 142. Bezprozvanny I, Watras J, Ehrlich BE, 1991. Bell-shaped calcium-response curves of Ins(1,4,5)P₃ and calcium gated channels from endoplasmic reticulum of cerebellum. Nature 351: 751-754. CrossRef PubMed
  2. 143. Foskett JK, White C, Cheung KH, Mak DOD, 2007. Inositol trisphosphate receptor Ca²⁺ release channels. Physiol Rev 87: 593-658. CrossRef PubMed PubMedCentral
  3. 144. Mak DO, McBride S, Foskett JK, 1998. Inositol 1,4,5-trisphosphate activation of inositol trisphosphate receptor Ca²⁺ channel by ligand tuning of Ca²⁺ inhibition. Proc Natl Acad Sci USA 95: 15821-15825. CrossRef PubMed PubMedCentral
  4. 145. Marchenko SM, Thomas RC, 2006. Nuclear Ca²⁺ signalling in cerebellar Purkinje neurons. Cerebellum 5: 36-42. CrossRef PubMed
  5. 146. Marchenko SM, Yarotskyy VV, Kovalenko TN, Kostyuk PG, Thomas RC, 2005. Spontaneously active and InsP₃-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurons. J Physiol 565: 897-910. CrossRef PubMed PubMedCentral
  6. 147. Zhang S-J, Zou M, Lu L, Lau D, Ditzel DAW, et al., 2009. Nuclear calcium signaling controls expression of a large gene pool: identification of a gene program for acquired neuroprotection induced by synaptic activity. PLoS Genet 5(8): e1000604. CrossRef PubMed PubMedCentral
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